Heart Of Darkness No Cd 11 [EXCLUSIVE]
LINK ::: https://urllio.com/2t7g8s
Annie feels the pit of anxiety in her stomach and notices her heart begins to beat faster. In response, she starts her familiar ritual of mentally counting to seven over and over again. She connects her counting to a physical action, like tapping her hand on the armrest of the couch seven times, clicking her heels together seven times, or moving her head to one side and back again seven times.
145 I call with all my heart;(ix) answer me, Lord, and I will obey your decrees.(iy)146 I call out to you; save me(iz) and I will keep your statutes.147 I rise before dawn(ja) and cry for help; I have put my hope in your word.148 My eyes stay open through the watches of the night,(jb) that I may meditate on your promises.149 Hear my voice(jc) in accordance with your love;(jd) preserve my life,(je) Lord, according to your laws.150 Those who devise wicked schemes(jf) are near, but they are far from your law.151 Yet you are near,(jg) Lord, and all your commands are true.(jh)152 Long ago I learned from your statutes(ji) that you established them to last forever.(jj)
161 Rulers persecute me(kb) without cause, but my heart trembles(kc) at your word.162 I rejoice(kd) in your promise like one who finds great spoil.(ke)163 I hate and detest(kf) falsehood but I love your law.(kg)164 Seven times a day I praise you for your righteous laws.(kh)165 Great peace(ki) have those who love your law, and nothing can make them stumble.(kj)166 I wait for your salvation,(kk) Lord, and I follow your commands.167 I obey your statutes, for I love them(kl) greatly.168 I obey your precepts(km) and your statutes,(kn) for all my ways are known(ko) to you.
Effect of green light during incubation on embryo and organs development. (A) Embryonic BW, as percentage of embryo weight and egg weight incubated under green light or kept in the dark on E16 and D1. (B) Liver, heart and leg muscle weight as percentage of BW of on E16. (C) Liver, heart and leg muscle weight as percentage of BW of embryos on D1. (D,E) Image of higher magnifications (magnified 60 times) of representative areas from the gastrocnemius muscle (left) under dark and green light. (F) The myofiber diameter of gastrocnemius muscle. The data are presented as mean ± SEM. * p < 0.05, ** p < 0.01. Abbreviations: dark group, 0G:24D; green light group, 12G:12D; E16, embryonic days 16. D1, day of hatching; BW, body weight.
Skeletal muscle development (myogenesis) is tightly controlled by muscle-specific myogenic regulatory factors such as MyoD, MYF5, MyoG, and MRF4. The expression of these factors is undetectable in quiescent satellite cells, but they are sequentially expressed when these cells are activated [30,43]. Satellite cells (myoblasts) under Pax3 and Pax7 regulation begin the myogenic program by expressing MYF5 and MyoD . When satellite cells are activated to generate daughter myogenic precursor cells, MyoG and MRF4 subsequently regulate terminal differentiation into myotubes . In contrast, MSTN negatively regulates skeletal muscle proliferation and differentiation and downregulates Pax 3, MyoD, MYF5 and MyoG expression [46,47]. The expression of MRF genes including MyoG and MRF4 is upregulated by IGF-1 administered to duck embryos in ovo , whereas Pax7, MYF5, and MyoD is upregulated, and MSTN expression is downregulated in embryonic chickens . We found significantly higher MyoG, MyoD, and MYF5 mRNA expression in leg muscles accompanied by upregulated IGF-1 production in embryos incubated under green light than darkness on E16 but not on D1. Moreover, green light stimuli upregulated MRF4 mRNA expression on D1. Green light during incubation also upregulates MyoG and MyoD mRNA expression, resulting in increased muscle fiber and chicken breast muscle development one week after hatching . This phenomenon indicates that green light promotes the proliferation and differentiation of skeletal muscle satellite cells during late embryogenesis, and that satellite cells start to proliferate before differentiation. In contrast, decreased MSTN expression under green light, confirmed its role in counteracting muscle growth. In addition, Pax3 and Pax7 mRNA expression was significantly increased under green light on D1. Paired box 7 is considered to be an early marker of myogenesis during post-hatching muscle growth . Therefore, these results suggest that monochromatic green light photostimulation results in a larger reservoir of myogenic progeny cells in newly hatched goslings, and further explains the stimulated growth of embryonic leg muscle and the larger myofiber diameter on D1.
Insulin-like growth factor 1 is mainly secreted in the liver, where GHRs are located in the liver, which is the connection between the somatotropic axis and green light photostimulation. Liver development was accelerated under green light. Energy metabolic pathways, such as gluconeogenesis, glycolysis, and glycogenesis, are upregulated in the liver during the final critical period of embryo development . Our transcriptome results showed that the expression of genes involved in muscle development, including muscle system process, muscle tissue morphogenesis, and muscle contraction, was induced by green light during incubation. In addition, green light influenced several metabolic pathways, including amino acid metabolism, the citrate cycle (TCA cycle), pyruvate metabolism, glycolysis, and gluconeogenesis. Asparagine synthetase (ASNS) is a key enzyme that catalyzes the synthesis of asparagine from aspartate . Both ASNS transcript and L-asparagine levels were downregulated by green light, resulting in an increased aspartate reserve in the liver, which might provide more substrate for pyruvate synthesis. As an intermediate product during the synthesis of acetyl-CoA from pyruvate , less s-acetyldihydrolipoamide-E was found in the liver under green light than darkness, indicating increased pyruvate production in the liver. Furthermore, the biochemical indices also confirmed these results, as pyruvate levels were increased by green light. The increase in hepatic glycogen and parallel change in serum glucose content suggest that green light enhances glucose synthesis/gluconeogenesis in the liver, thereby increasing glucose content.
The same number of Yangzhou goose eggs were assessed in three consecutive incubations. We collected 1536 normal eggs (average weight, 143.33 ± 5.43 g) from 40-week-old Yangzhou geese (male: female = 1:5) and analyzed 512 eggs per experiment. The eggs were stored no more than 3 d with a temperature of 20°C. The front windows of two incubators (FHQ-V1, Hengxin Incubation Equipment Co., Ltd., Nanjing, China) were covered with opaque sheets to prevent light intrusion from outside. One incubator was fitted with strips of monochromatic green LEDs (NVC Lighting Co., Ltd., Guangzhou, China), with a 12 h green light/12 h dark cycle (12G:12D) during the first 28 days of incubation. We eliminated light-induced egg overheating using a mechanical timer to control 24 green-light cycles of 0.5 h of darkness followed by 0.5 h of light. Light intensity at the egg level was adjusted to 200 lx using a digital luxmeter. Eggs in the other incubator remained in complete darkness (0G:24D). Thereafter, 512 eggs were equally assigned to the same location in eight trays per incubator and every two trays were replicates. 2b1af7f3a8